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In addition, various projects have been concerned with the curation of the genic regions of these genomes, most notably RefSeq. Included are the genomes of human, mouse, Norwegian brown rat, fruit fly, fugu, chimpanzee, and zebrafish. As of December 24, 2004, the Genomes OnLine Database lists 1,245 on-going or completed genome projects. Recent advances in DNA sequencing technologies have resulted in the availability of the assembly of a number of genomes at various stages.
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In addition, sequence similarity for a set of oligonucleotides constructed for the same set of genes indicates high specificity in oligo design. These results indicate MPrime accurately incorporates standard PCR primer design characteristics to produce high scoring primer pairs for genes of interest. A set of products created for mouse housekeeping genes from MPrime-designed primer pairs has been validated using both PCR-amplification and DNA sequencing. We have developed an integrated system, MPrime, in order to efficiently calculate primer pairs or specific oligonucleotides for multiple genic regions based on a keyword, gene name, accession number, or sequence fasta format within the rat, mouse, human, fruit fly, and zebrafish genomes. Therefore, to efficiently study a number of gene products associated with a disease, pathway, or other biological process, it is necessary to be able to design primer pairs or oligonucleotides en masse rather than using a time consuming and laborious gene-by-gene method. The availability of large-scale genomic and genic sequence data coupled with advances in microarray technology have made it possible to study the expression of large numbers of sequence products under several different conditions in days where traditional molecular biology techniques might have taken months, or even years.
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Enhancements in sequencing technology have recently yielded assemblies of large genomes including rat, mouse, human, fruit fly, and zebrafish.